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KMID : 1007520140230062007
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Food Science and Biotechnology
2014 Volume.23 No. 6 p.2007 ~ p.2012
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Molecular Cloning and Expression of Amylosucrase from Highly Radiation-resistant Deinococcus radiopugnans
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Kim Myo-Deok
Seo Dong-Ho
Jung Jong-Hyun
Jung Dong-Hyun
Joe Min-Ho
Lim Sang-Yong
Lee Ju-Hoon
Park Cheon-Seok
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Abstract
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Amylosucrase (AS), a glucosyltransferase of the glycoside hydrolase 13 family, does not require a nucleotide-activated sugar as a glucosyl-donor. A gene (drpas) of Deinococcus radiopugnans encoding a putative AS was identified and cloned for characterization. The amino acid sequence revealed that drpas exhibited 76 and 74% identities with AS genes from D. geothermalis and D. radiodurans, respectively. Recombinant DRpAS exhibited AS activities. The ratios of hydrolysis, polymerization, and isomerization reactions were 5.8:82.7:11.5 at 40oC. The DRpAS was highly thermostable and produced more polymerization products than AS from D. radiodurans although the optimum growth temperature of both strains is 30oC.
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KEYWORD
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amylosucrase, Deinococcus radiopugnans, glycoside hydrolase 13 family (GH13), insoluble glucan, transglycosylation
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